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以牛血清白蛋白为中间载体,将血卟啉衍生物(HPD)与抗胃癌单克隆抗体3H11交联。交联物3H11-BSA-HPD的克分子比为1:1:200。本文对3H11-BAS-HPD的体内外抗肿瘤作用进行了研究,并与直接交联物3H11-HPD进行比较。3H11-BSA-HPD和3H11-HPD对胃癌靶细胞BGC-823的细胞毒效应相似,并均明显比游离HPD强。在接种靶细胞(2×10~5细胞/只)的裸鼠中,对照组和HPD组均于接种后13天内形成瘤块,而间接和直接交联物处理组在34天实验期内仅有1/6动物形成肿瘤。结果表明3H11-BSA-HPD和3H11-HPD在HPD相等剂量下,具有相似的导向杀伤肿瘤细胞的作用。
Using bovine serum albumin as an intermediate carrier, hematoporphyrin derivative (HPD) was cross-linked with anti-gastric cancer monoclonal antibody 3H11. The cross-linked product 3H11-BSA-HPD had a molar ratio of 1:1:200. In this paper, the antitumor effects of 3H11-BAS-HPD in vivo and in vitro were studied and compared with the direct crosslinker 3H11-HPD. The cytotoxic effects of 3H11-BSA-HPD and 3H11-HPD on gastric cancer target cell BGC-823 were similar, and all were significantly stronger than free HPD. In nude mice inoculated with target cells (2 x 10~5 cells/body), the control group and the HPD group all formed tumor masses within 13 days after inoculation, whereas the indirect and direct cross-linker treatment groups were only observed during the 34-day experimental period. One in six animals develop tumors. The results showed that 3H11-BSA-HPD and 3H11-HPD had similar directed killing effect on tumor cells at equivalent doses of HPD.