采用同步辐射X射线荧光分析(SRXRF)与亚细胞分布研究相结合的方法,从细胞组织微区及亚细胞分布层面首次揭示了药用植物三七(Panax notoginseng)受砷(As)的毒害作用。研究结果表明,三七根部的As元素多集中在表皮组织中,且有向维管束运转的趋势;细胞液是As主要富集的亚细胞组分,用20 mg·L~(-1)As处理的三七细胞液中As含量约为不加As的200倍;分析三七主根亚细胞组分的As含量与营养液As浓度的曲线拟合方程,确定了营养液As浓度直接影响细胞液组分的As含量;各组分所占比例从大到小表现为:细胞液>细胞壁>细胞质,20 mg·L~(-1)As处理的细胞液中砷含量约占三组分总量的65.78%,达到最高比例,细胞壁和细胞器中则始终维持较低的砷浓度。 Using the method of synchrotron radiation X-ray fluorescence (SRXRF) and subcellular distribution, the toxic effect of As (As) on the Panax notoginseng plant was first revealed from the micro and subcellular distribution of the cells. . The results showed that the As elements in the root of Panax notoginseng were mostly concentrated in the epidermis and tended to migrate to the vascular bundle. The cytosol was the main enrichment subunit of As with 20 mg · L -1 As The content of As in treated Panax notoginseng was about 200 times higher than that of As, and the curve fitted equation of As content in Panax notoginseng subunit and As concentration in nutrient solution was analyzed. It was confirmed that the concentration of As As content of the components; the proportion of each component from large to small as: cell solution> cell wall> cytoplasm, 20 mg · L -1 As treatment cytosol of arsenic content of about three components of total Of the 65.78%, reaching the highest proportion, the cell wall and organelles always maintain a low concentration of arsenic.