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目的探讨EphA2与其配体EphrinA1在胃癌组织中的表达及与肿瘤血管生成的关系。方法利用免疫组织化学法检测82例胃癌手术切除标本中癌组织、癌旁组织及正常胃黏膜中EphA2、EphrinA1的表达,并采用CD34抗体标记微血管内皮细胞,计算微血管密度(MVD)。分析EphA2、EphrinA1的表达与MVD之间的相关性及其与胃癌临床病理特征的关系。结果胃癌组织中EphA2、EphrinA1的表达及MVD显著高于癌旁组织及正常胃黏膜(P<0.01);EphA2、Eph-rinA1表达水平及MVD值与胃癌的分化程度、浸润深度及淋巴结转移有关(P<0.05),MVD值还与肿瘤直径有关(P<0.05);Spearman等级相关分析表明,EphA2、EphrinA1表达分别与MVD呈显著正相关(r=0.485,P<0.01;r=0.512,P<0.01)。结论EphA2及其配体EphrinA1在胃癌组织中特异性高表达,可能参与肿瘤血管的生成,在胃癌的发病及恶性进展中发挥重要作用。
Objective To investigate the expression of EphA2 and its ligand EphrinA1 in gastric carcinoma and its relationship with tumor angiogenesis. Methods Immunohistochemistry was used to detect the expression of EphA2 and EphrinA1 in 82 gastric cancer specimens, adjacent tissues and normal gastric mucosa. Microvessel density (MVD) was calculated using CD34 antibody. To analyze the correlation between EphA2, EphrinA1 expression and MVD and its relationship with clinicopathological characteristics of gastric cancer. Results The expressions of EphA2 and EphrinA1 and MVD in gastric cancer tissues were significantly higher than those in paracancer tissues and normal gastric mucosa (P <0.01). The expressions of EphA2, Eph-rinA1 and MVD were correlated with the differentiation, depth of invasion and lymph node metastasis (P <0.05). The MVD also correlated with tumor diameter (P <0.05). Spearman rank correlation analysis showed that there was a significant positive correlation between EphA2 and EphrinA1 expression and MVD (r = 0.485, 0.01). Conclusion EphA2 and its ligand EphrinA1 are highly expressed in gastric cancer tissues and may be involved in the generation of tumor blood vessels. They play an important role in the pathogenesis and malignant progression of gastric cancer.