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目的:运用代谢组学方法研究结扎冠状动脉致大鼠急性心肌缺血和三七总皂苷给药后大鼠血清代谢物组变化。方法:通过GC-MS技术分析正常组、急性心肌缺血造模组和三七总皂苷给药组的大鼠血清代谢物组,进而使用主成分分析(PCA)和偏最小二乘法-判别(PLS-DA)法研究3组代谢物谱的差异。结果:PCA和PLS-DA模型对不同组大鼠血清代谢物组预测分类效果良好;采用t检验和变量重要性投影法(VIP)挑选得到血清中10种潜在代谢标志物。结论:代谢组学方法可整体上反映生物体的病理生理状态,有望用于中药药效评价。
OBJECTIVE: To study the changes of serum metabolites in rats with acute myocardial ischemia induced by ligation of coronary artery and the administration of total saponins of Panax notoginseng by means of metabolomics. Methods: Serum metabolites of rats in normal group, acute myocardial infarction model group and Panax notoginseng saponin group were analyzed by GC-MS, and then the principal component analysis (PCA) and partial least squares PLS-DA) method to study the difference of metabolites in the three groups. Results: The PCA and PLS-DA models had a good effect on predicting the serum metabolites of different groups. Ten potential serum metabolites were obtained by t-test and variable-importance projection (VIP). Conclusion: Metabolomics method can reflect the pathophysiological state of the organism as a whole, and is expected to be used for evaluating the efficacy of traditional Chinese medicine.