论文部分内容阅读
目的探讨转移抑制BRMS1基因对人直肠癌裸鼠成瘤性以及抑制肿瘤细胞远处转移的影响。方法通过将BRMS1基因导入人直肠癌细胞株LOVO中,建立裸鼠实验性转移模型和自发性转移模型,观察转染组(A组)、空载体组(B组)、未转染组(C组)裸鼠的生长状态和肿瘤远处转移状况。结果自发性转移模型:A1组转染组细胞、B1组转染空载体组细胞和C1组转染组癌细胞接种后裸鼠的体质量及肿瘤平均直径无统计学差异(P>0.05)。实验性转移模型:转染空载体组B2和未转染组C2的裸鼠肺脏转移率为100%;转染组A2组肺脏和肝脏转移灶的病理分级标准较B2组和C2组降低,A2组肺转移分级以II级为主,B2组、C2组以II级、III级为主;A2组未见肝转移灶,而B2组、C2组肝转移分级以I级、II级为主。结论 BRMS1基因能够明显抑制直肠癌LOVO细胞的远处转移的能力,但并不影响肿瘤细胞本身的生长,提示BRMS1基因可能成为抑制直肠癌远处转移的新靶点。
Objective To investigate the effect of metastasis suppressor BRMS1 gene on the tumorigenicity of human rectal cancer in nude mice and its effect on the distant metastasis of tumor cells. Methods The BRMS1 gene was transfected into human ovarian cancer cell line LOVO to establish experimental metastasis model and spontaneous metastasis model in nude mice. The transfection group (group A), empty vector group (group B), untransfected group (C) Group) nude mice growth status and tumor distant metastasis. Results In the spontaneous metastasis model, there was no significant difference in body mass and average diameter of tumor between A1 group transfected cells, empty vector transfected group B1 and C1 transfected cancer cells (P> 0.05). Experimental metastasis model: The lung metastasis rate of lung in nude mice transfected with empty vector group B2 and untransfected group C2 was 100%. The pathological grading standards of lung and liver metastases in A2 group were lower than those in B2 and C2 groups The grade of lung metastasis was mainly grade II, while those of group B2 and C2 were mainly grade II and grade III. There was no liver metastasis in group A2, but grade I and grade II were predominant in group B2 and C2. Conclusion The BRMS1 gene can significantly inhibit the distant metastasis of rectal cancer LOVO cells, but does not affect the growth of tumor cells, suggesting BRMS1 gene may be a new target of inhibiting the distant metastasis of rectal cancer.