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目的:观察非小细胞肺癌(NSCLC)外周血淋巴细胞亚群及Treg细胞水平变化。方法:选择NSCLC 80例为观察组,在我院参加健康体检者60例为对照组。采用流式细胞仪技术检测两组外周血T淋巴细胞亚群占外周血单个核细胞的比例;对于NSCLC病例按照不同临床分期、不同病理类型及不同分化程度进行分组,检测并比较各组外周血T淋巴细胞亚群占外周血单个核细胞的比例。结果:两组CD3+T细胞、CD4+T细胞、CD8+T细胞、NK细胞、B细胞占外周血单个核细胞的比例差异均不显著(P>0.05);观察组Treg细胞显著高于对照组(P<0.05)。临床分期Ⅰ-Ⅱ期组与Ⅲ-Ⅳ期组以及分化程度高中分化组与低分化组之间,CD3+T细胞、CD4+T细胞、CD8+T细胞、NK细胞和B细胞占外周血单个核细胞比例差异均不显著(P>0.05);Ⅲ-Ⅳ期组Treg细胞所占比例显著高于Ⅰ-Ⅱ期组,低分化组Treg细胞显著高于高中分化组(P<0.05)。鳞癌组、腺癌组和其他病理类型组CD3+T细胞、CD4+T细胞、CD8+T细胞、NK细胞、B细胞及Treg细胞占外周血单个核细胞比例均差异不显著(P>0.05)。结论:Treg细胞检测对于判定NSCLC免疫功能具有重要意义。
Objective: To observe the changes of peripheral blood lymphocyte subsets and Treg cells in non-small cell lung cancer (NSCLC). Methods: 80 cases of NSCLC were selected as the observation group, 60 cases of healthy physical examination in our hospital as the control group. Flow cytometry was used to detect the percentage of peripheral T-lymphocyte subsets in peripheral blood mononuclear cells in two groups. The NSCLC patients were divided into different clinical stages, different pathological types and different degrees of differentiation. The peripheral blood T lymphocyte subsets accounted for the proportion of peripheral blood mononuclear cells. Results: The percentage of CD3 + T cells, CD4 + T cells, CD8 + T cells, NK cells and B cells in peripheral blood mononuclear cells were not significantly different (P> 0.05). Treg cells in the observation group were significantly higher than those in the control Group (P <0.05). CD3 + T cells, CD4 + T cells, CD8 + T cells, NK cells and B cells in clinical stage Ⅰ-Ⅱ group and Ⅲ-Ⅳ group as well as between high differentiated group and poorly differentiated group accounted for a significant proportion of peripheral blood mononuclear cells (P> 0.05). The proportion of Treg cells in stage Ⅲ-Ⅳ was significantly higher than that in stage Ⅰ-Ⅱ, and the level of Treg cells in poorly differentiated group was significantly higher than that in high-differentiated group (P <0.05). The percentage of CD3 + T cells, CD4 + T cells, CD8 + T cells, NK cells, B cells and Treg cells in peripheral blood mononuclear cells were not significantly different between squamous cell carcinoma, adenocarcinoma and other pathological types (P> 0.05 ). Conclusion: The detection of Treg cells is of great significance for judging the immune function of NSCLC.