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应用抗胶质纤维酸性蛋白（ＧＦＡＰ）、抗牛Ｓ－１００蛋白（Ｓ－１００）、抗波形蛋白（Ｖｉｍｅｎｔｉｎ）、抗神经特异性烯醇化酶（ＮＳＥ）、抗癌胚抗原（ＣＥＡ）及抗人角蛋白（Ｋｅｒａｔｉｎ）免疫血清，采用ＡＢＣ法（卵白素－生物素－过氧化物酶复合物法），分别对５５例颅内肿瘤（原发神经组织肿瘤４９例，转移癌６例）进行检测，并进行瘤细胞异质性的研究。结果显示，在同一时瘤组织内免疫染色阳性细胞在质与量的分布上极不均匀；瘤细胞免疫染色反应与肿用的组织学分红或／及细胞生长率（核分裂计数）之间未见明显相关。研究提示：（１）抗元ＧＦＡＰ、Ｓ－１００、Ｖｉｍｅｎｔｉｎ、ＮＳＥ、ＣＥＡ及Ｋｅｒａｔｉｎ血清的免疫染色在颅内肿瘤的诊断与鉴别诊断上极有价值；（２）免疫在型异质性所显示的肿瘤细胞亚群与病理组织学分级并不完全相同，要获得肿瘤的正确诊断与分类，从多处瘤组织取材及多种抗体免疫染色是必要的；（３）颅内肿瘤细胞免疫组织化学异质性的生物学意义有待阐明。
Anti-glial fibrillary acidic protein (GFAP), anti-bovine S-100 protein (S-100), anti-vimentin, anti-neuron specific enolase (NSE) Fifty-five cases of intracranial tumors (49 cases of primary nerve tissue and 6 cases of metastatic carcinoma) were treated with Keratin immune serum by ABC method (avidin-biotin-peroxidase complex method) Detection, and tumor cell heterogeneity research. The results showed that at the same time in the tumor tissue immunostaining positive cells in the quality and quantity distribution is extremely uneven; tumor cells immunostaining reaction and swollen histological dividend and / or cell growth rate (mitotic count) between the Obviously related. The research suggests that: (1) immunostaining of anti-GFAP, S-100, Vimentin, NSE, CEA and Keratin sera is of great value in the diagnosis and differential diagnosis of intracranial tumors; (2) Of tumor cell subsets and pathological histological grading is not exactly the same, to obtain the correct diagnosis and classification of tumors, from multiple tumor tissue and a variety of antibody immunostaining is necessary; (3) intracranial tumor cell immunohistochemistry The biological significance of heterogeneity remains to be elucidated.