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目的本研究从蛋白水平分析Mel-18、Notch-1和PLK-1在不同阶段乳腺组织中的表达情况,探讨三者在乳腺癌发生、发展中可能的作用。方法收集正常乳腺组织、导管上皮不典型增生及乳腺癌标本共176例,采用罗氏全自动免疫组化染色法检测Mel-18、Notch-1和PLK-1在不同乳腺组织中的表达,比较其差异并分析Mel-18、Notch-1和PLK-1在不同乳腺疾病中的表达情况以及与主要病理学特征的关系。结果①Mel-18在正常乳腺组织中的表达明显高于乳腺癌,差异显著(P<0.05);Notch-1和PLK-1在乳腺癌中的表达要明显高于正常乳腺组织(P<0.05)。②Notch-1的过表达与组织学分级、临床分期及淋巴结转移呈正相关(P<0.05),与年龄和肿瘤直径之间无明显相关性(P>0.05)。PLK-1的表达仅与肿瘤的组织分级呈正相关(P<0.05),与患者年龄、肿瘤直径、临床分期及淋巴结转移之间无明显相关性(P>0.05)。结论 Mel-18为乳腺癌的肿瘤抑制基因,由于乳腺癌中Notch-1通路的激活使得乳腺增殖加速,细胞的分化状态降低。PLK-1蛋白的过量表达引起乳腺细胞的分裂和增殖,促进了乳腺癌的形成。联合检测3个指标对判定乳腺癌的预后有重要意义。
OBJECTIVE: To investigate the expression of Mel-18, Notch-1 and PLK-1 in different stages of breast cancer from the protein level, and to explore the possible roles of these three in the development and progression of breast cancer. Methods Totally 176 cases of normal breast tissue, atypical hyperplasia of ductal epithelium and breast cancer were collected. The expression of Mel-18, Notch-1 and PLK-1 in different breast tissues were detected by Roche immunohistochemistry. Differences and analyze the expression of Mel-18, Notch-1 and PLK-1 in different breast diseases and the relationship with the main pathological features. Results ① The expression of Mel-18 in normal breast tissue was significantly higher than that in breast cancer (P <0.05). The expression of Notch-1 and PLK-1 in breast cancer tissues was significantly higher than that in normal breast tissues (P <0.05) . Overexpression of Notch-1 was positively correlated with histological grade, clinical stage and lymph node metastasis (P <0.05), but not with age and tumor diameter (P> 0.05). There was no significant correlation between the expression of PLK-1 and tumor grade (P <0.05), and age, tumor diameter, clinical stage and lymph node metastasis (P> 0.05). Conclusion Mel-18 is a tumor suppressor gene in breast cancer. Due to the activation of Notch-1 pathway in breast cancer, the proliferation of mammary gland is accelerated and the differentiation state of cells is decreased. Overexpression of PLK-1 protein causes division and proliferation of breast cells and promotes the formation of breast cancer. Joint detection of three indicators of the prognosis of breast cancer is of great significance.