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目的 :在体实验研究神经生长因子 (NGF)对部分视神经损伤的保护效应。方法 :建立视神经不完全损伤模型 ,球后注射神经生长因子 ,记录F VEP ,定量RGCs和髓鞘以评价治疗效果。结果 :与正常组相比 ,NGF组F VEP波形平缓 ,N1和P1潜伏期延长 ,但无显著性差异 ,N1-P1、P1-N1幅值降低、N2 潜伏期延长 ,有显著性差异。NGF对神经节细胞挽救率是19 3%。NGF组未发生溃变的轴突数是损伤未治疗组的 1 83倍。结论 :NGF可挽救视网膜神经节细胞的存活率 ,减少轴突溃变 ,对视网膜神经节细胞继发变性有明显的保护作用。
Objective: To study the protective effect of nerve growth factor (NGF) on part of optic nerve injury in vivo. Methods: The model of incomplete optic nerve injury was established. After the ball injection of nerve growth factor, F VEP, RGCs and myelin were quantified to evaluate the therapeutic effect. Results: Compared with the normal group, the waveform of F VEP in NGF group was gentle and the latency of N1 and P1 was prolonged, but there was no significant difference. The amplitude of N1-P1, P1-N1 decreased and the latency of N2 prolonged with significant difference. NGF ganglion cell salvage rate was 19 3%. The number of non-degenerated axons in the NGF group was 183 times that of the untreated group. Conclusion: NGF can save the survival rate of retinal ganglion cells, reduce axonal degeneration, retinal ganglion cell degeneration secondary obvious protective effect.