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目的:建立以柱层析-高效液相色谱法测定阳强保肾丸中淫羊藿苷含量的方法。方法:色谱柱为十八烷基硅烷键合硅胶(200mm×4·6mm,10μm),流动相为0·075mol·L-1磷酸溶液-乙氰(73∶27),检测波长为270nm。结果:淫羊藿苷检测浓度在10~18μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0·9995);平均加样回收率为94·35%,RSD=1·12%(n=6)。结论:本法简便、准确、重现性好,可用于阳强保肾丸的质量控制。
Objective: To establish a method for determination of icariin content in Yangqiangbaoshen Pills by column chromatography-high performance liquid chromatography. Method: The chromatographic column was octadecylsilane-bonded silica gel (200mm×4.6mm, 10μm), the mobile phase was 0·075mol·L-1 phosphoric acid solution-acetonitrile (73∶27), detection wavelength was 270nm. RESULTS: There was a good linear relationship between the concentration of icariin in the range of 10-18 μg·mL-1 and the integrated value of the peak area (r=0·9995); the average recovery rate of the icariin was 94.35 %, RSD=1· 12% (n=6). Conclusion: This method is simple, accurate and reproducible. It can be used for the quality control of Yangqiangbaoshen pill.