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目的观察螺内酯对T2DM大鼠肾脏紧张素转换酶2(ACE2)和细胞间黏附分子(ICAM-1)表达的影响,以探讨其对糖尿病肾脏病变的保护作用。方法用高脂高糖饲料喂养大鼠2个月后,腹腔注射30mg/kg STZ制备糖尿病大鼠模型,分为正常对照(NC)组、糖尿病(DM)组、螺内酯治疗(R)组,检测第16周FBG、Cr、24hUAER、肾重/体重(×10-3);并用免疫组化和RT-PCR的方法检测16周后大鼠肾脏ACE2和ICAM-1表达的改变。结果第16周末DM、R组各项比较,差异无统计学意义(P>0.05),但均高于NC组(P均<0.01);R组ACE2水平高于DM组(P<0.01),ICAM-1水平低于DM组(P<0.05)。结论螺内酯通过增加肾脏组织ACE2的表达、降低ICAM-1的表达而发挥保护肾脏的作用。
Objective To investigate the effects of spironolactone on the expression of renal ACE2 and ICAM-1 in T2DM rats and its protective effects on diabetic nephropathy. Methods Rats were fed with high-fat and high-sugar diet for 2 months. The diabetic rats were injected intraperitoneally with 30mg / kg STZ and divided into normal control (NC), diabetic (DM) and spironolactone (R) (FBG, Cr, 24hUAER, kidney weight / body weight (× 10-3) at week 16). The expressions of ACE2 and ICAM-1 in kidney were detected by immunohistochemistry and RT-PCR after 16 weeks. Results There was no significant difference between the DM and R groups at the end of the 16th week (P> 0.05), but both were higher than those in the NC group (P <0.01). The ACE2 level in the R group was higher than that in the DM group (P <0.01) ICAM-1 levels were lower in the DM group (P <0.05). Conclusion Spironolactone can protect the kidneys by increasing the expression of ACE2 in the kidney and decreasing the expression of ICAM-1.