论文部分内容阅读
目的探讨全反式维甲酸(ATRA)对阿霉素肾病大鼠足细胞是否具有保护作用。方法通过建立阿霉素肾病大鼠模型,随机分为三组,即对照组、模型组、治疗组,于阿霉素注射后第2天治疗组每天给予20 mg/kg ATRA灌胃,每周检测24-h尿蛋白定量,4周后处死大鼠,检测血清白蛋白、胆固醇、甘油三酯、血肌苷、尿素氮、nephrin的表达。结果治疗组与模型组相比大鼠尿蛋白明显减少(P<0.01)。治疗组nephrin蛋白和mRNA含量均较模型组有所增加(P<0.05)。结论 ATRA对阿霉素肾病大鼠足细胞有保护作用,其机制部分与nephrin的表达改变有关。
Objective To investigate whether all-trans retinoic acid (ATRA) can protect podocytes of adriamycin-induced nephropathy rats. Methods The rat model of adriamycin nephropathy was established and randomly divided into three groups: control group, model group and treatment group. The treatment group received 20 mg / kg ATRA daily on the second day after doxorubicin injection, The 24-h urinary protein was quantified. After 4 weeks, the rats were sacrificed and the serum albumin, cholesterol, triglyceride, serum creatine, urea nitrogen and nephrin were detected. Results Compared with model group, urinary protein in treatment group decreased significantly (P <0.01). Nephrin protein and mRNA levels in the treatment group were increased compared with the model group (P <0.05). Conclusion ATRA has a protective effect on podocytes of adriamycin-induced nephropathy rats, and its mechanism is partly related to the alteration of nephrin expression.