本工作应用热激蛋白70KD(hsp 70)核酸分子杂交方法,检测了:1.自发性高血压(SHR)主动脉和离体培养的主动脉平滑肌细胞受热激后hsp 70mRNA水平的变化;2.不同细胞培养时间(3个月与6周)的SHR、WKY主动脉hsp 70mRNA水平。结果提示SHR主动脉hsp mRNA水平增加,SHR细胞培养受热激(42℃,15min)后2h,hsp 70mRNA水平明显高于WKY鼠者,6周较3个月的SHR细胞hsp 70mRNA水平高;6周的SHR细胞较同期和3个月的WKY细胞hsp 70mRNA高。推论SHR血管平滑肌细胞对热敏感,原癌基因c-myc和抗癌基因p~(53)可能参与hsp 70表达调控,并共同参与SHR细胞增殖的调节。 In this study, the heat shock protein 70KD (hsp70) hybridization method was used to detect the changes of hsp70mRNA in aortic and in vitro cultured aortic smooth muscle cells of spontaneously hypertensive rats (SHR) .2. SHR and WKY aortic hsp 70 mRNA levels in different cell culture periods (3 and 6 weeks). The results showed that the level of hsp mRNA in the aorta of SHR increased. The level of hsp 70 mRNA in SHR cells was significantly higher than that in WKY mice at 2 h after heat shock (42 ℃, 15 min) Of SHR cells were higher than that of WKY cells in the same period and 3 months. It is concluded that SHR vascular smooth muscle cells are sensitive to heat. The proto-oncogene c-myc and anti-oncogene p ~ (53) may be involved in the regulation of hsp 70 expression and participate in the regulation of SHR cell proliferation.