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本实验用相差显微电影、溶酶体荧光定位和透射电镜等方法,研究了大鼠离体肺泡巨噬细胞吞噬煤尘的作用,并用二氧化矽和酵母菌作实验对照。结果表明,煤尘颗粒同二氧化矽类似,经过附着,迅速以吞噬体形式被摄入胞浆,而酵母菌呈典型的伪足包围和缓幔吞噬过程。实验证明,用吖啶橙作活细胞溶酶体定位是有效的。被标记的溶酶体发出强橙色荧光。当煤粒吞噬体进入胞浆后,常被多个溶酶体接触包围,形成次级溶酶体。电镜观察提示,煤尘可引起次级溶酶体膜的损害。
In this study, phase contrast micro-film, lysosome fluorescence localization and transmission electron microscopy and other methods to study the rat alveolar macrophage phagocytosis of coal dust, and silicon dioxide and yeast for experimental control. The results showed that coal dust particles similar to silicon dioxide, after attachment, quickly in the form of phagosomes were taken into the cytoplasm, and yeast were typical pseudopods surrounded by slow mantle phagocytosis process. Experiments show that using acridine orange as live cell lysosome localization is effective. Labeled lysosomes emit a strong orange fluorescence. When the coal phagosomes into the cytoplasm, often surrounded by multiple lysosome contacts, the formation of secondary lysosomes. Electron microscopy suggests that coal dust can cause secondary lysosomal membrane damage.