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为了探索人体肝癌的发病机理,了解肝癌细胞的生物学特性,为防治肝癌提供不同类型的肝脏细胞模型,我们在中国科学院上海细胞生物学研究所的指导和帮助下,自1975年以来,开展了人体肝脏细胞的培养工作。一年来,我们一共培养了11例肝脏细胞,部份细胞至今生长稳定,代代相传,形态比较一致。其中一株肝癌传代细胞(QGY7703),已进行了生物学特性的指标测定。培养方法肝脏标本除了正常肝、胚胎肝从尸解中获得以外,其余均从肝脏手术中取得。标本以无菌方法采取,浸人含有青霉素(200u/ml)和卡那霉素(200μg/ml)培养液中,在短时间内迅速接种培养。其接种过程为:先用Hank氏液反复洗涤,挑选良好的组织,用眼科剪刀剪成1mm~3小块,继以含有20％小牛血清的RPMI1640培养液冲洗二遍,随即接种于“小北京”
In order to explore the pathogenesis of human hepatocellular carcinoma, to understand the biological characteristics of hepatocellular carcinoma cells, and to provide different types of hepatocyte models for the prevention and treatment of liver cancer, we undertook the guidance and assistance of the Shanghai Institute of Cell Biology, Chinese Academy of Sciences, since 1975. Human liver cell culture work. Over the past year, we have cultivated a total of 11 liver cells. Some of the cells have grown steadily and passed on from generation to generation. One of the hepatoma-passaging cells (QGY7703) has been tested for biological characteristics. Culture methods Liver specimens were obtained from liver surgery except for normal liver and embryonic liver obtained from autopsy. Specimens were taken aseptically and immersed in culture medium containing penicillin (200 u/ml) and kanamycin (200 μg/ml) and inoculated in a short time. The inoculation process consisted of repeated washing with Hank’s solution, selecting a good tissue, cutting into 1 mm to 3 small pieces with an ophthalmic scissors, and then washing it twice with RPMI 1640 culture medium containing 20% calf serum, and inoculating it immediately. Beijing"