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目的探讨脂肪细胞因子抵抗素(resistin)对卵巢癌细胞系HO-8910细胞增殖的影响及初步表达机制。方法 2011年5-10月于中国医科大学中心实验室分别采用噻唑蓝(MTT)比色法,检测不同浓度的抵抗素对卵巢癌HO-8910细胞增殖能力的影响;利用si RNA敲除脂肪细胞中的抵抗素,转移条件培养基培养卵巢癌细胞HO-8910,通过MTT法检测细胞增殖情况;利用免疫印迹法(western blot)检测抵抗素作用于HO-8910细胞后m TOR及其下游靶蛋白S6总蛋白的磷酸化蛋白量的变化。结果抵抗素可以呈时间、剂量依赖性地促进HO-8910细胞的增殖,30~100μg/L抵抗素作用24h即可以显著促进HO-8910细胞的增殖,48h的作用更为明显(P<0.05);脂肪细胞条件培养基可显著促进HO-8910细胞的增殖,如果在脂肪细胞培养过程中给予特异性敲低抵抗素,则作用反之;抵抗素作用卵巢癌HO-8910细胞24h后m TOR及其下游靶蛋白S6的磷酸化水平显著增加。结论抵抗素对人卵巢癌细胞系HO-8910细胞的增殖能力有明显的促进作用,该作用机制可能与m TOR信号通路有关,可为诊治卵巢癌提供新的靶点。
Objective To investigate the effects of resistin on the proliferation of ovarian cancer cell line HO-8910 and its primary expression mechanism. METHODS: MTT assay was used to determine the effect of different concentrations of resistin on the proliferation of HO-8910 ovarian cancer cells from May to October in 2011. The knockdown of adipocytes HO-8910 cells were cultured with resistin and metastatic medium, and cell proliferation was detected by MTT assay. Western blotting was used to detect the expression of mTOR and its downstream target protein in HO-8910 cells Changes in the amount of phosphoprotein of S6 total protein. Results Resistin could promote the proliferation of HO-8910 cells in a time-and dose-dependent manner. The proliferation of HO-8910 cells was promoted by 30 ~ 100μg / L resistin for 24h, and the effect was more obvious at 48h (P <0.05) ; Adipocyte conditioned medium can significantly promote the proliferation of HO-8910 cells, if given in the process of adipocyte specific knockdown of resistin, the effect on the contrary; resistin effect ovarian cancer HO-8910 cells after 24h m TOR and its The phosphorylation level of downstream target protein S6 is significantly increased. CONCLUSION: Resistin may promote the proliferation of human ovarian cancer cell line HO-8910. The mechanism may be related to the mTOR signaling pathway, which may provide a new target for the diagnosis and treatment of ovarian cancer.