本文用自建的硫代黄素荧光染色法对不同年龄的小鼠胸腺细胞和健康人外周血T细胞分别进行了实验研究,经用此一种荧光颜料染色,可使小鼠胸腺细胞和人外周血T细胞呈现六种不同的荧光颜色。通过A花环试验发现形成A花环的有三种细胞都是对考地松敏感的细胞系。本染色法不仅可以区分T细胞的不同亚群,而且对这些亚群在免疫反应过程中的作用亦提供了一个较好的形态学观察方法。 In this paper, we used the self-built thioflavin fluorescence staining method to study the thymocytes of healthy mice and the peripheral blood T cells of healthy people respectively. After staining with this kind of fluorescent pigment, we can make mouse thymocytes and humans. Peripheral blood T cells exhibit six different fluorescent colors. Through the A garland test, it was found that three types of cells forming A rosettes were cell lines sensitive to Kodson. This staining method can not only distinguish different subpopulations of T cells, but also provide a better morphological observation method for the role of these subpopulations in the immune response process.