分离检测脑内递质氨基酸的含量,是研究脑代谢和脑功能活动的一个重要指标。本文应用高效液相色谱——柱后衍生法对大鼠脑内三个部位游离氨基酸进行了分离并对GABA(γ—氨基丁酸)、Tau(牛磺酸)、Gly(甘氨酸)、Glu(谷氨酸)和ASP(天冬氨酸)进行了定量。结果表明:随年龄增长,脑内抑制性递质GABA,Tau和Gly的含量降低或不变,兴奋性递质Glu和ASP含量增高或不变。并且还讨论了不同脑区GABA/Glu以及抑制性递质氨基酸总量与兴奋性递质氨基酸总量比值。 Separation and detection of brain amino acid content of neurotransmitters, is to study brain metabolism and brain function of an important indicator. In this paper, free amino acids in three parts of rat brain were isolated by high performance liquid chromatography-column derivatization and the effects of GABA (gamma-aminobutyric acid), Tau (taurine), Gly Glutamic acid) and ASP (aspartic acid) were quantified. The results showed that the contents of GABA, Tau and Gly in the brain decreased or remained unchanged with the increase of age, while the contents of Glu and ASP in the neurotransmitter increased or remained unchanged. And also discusses the different brain regions of GABA / Glu and inhibitory transmitter amino acid total and excitatory transmitter amino acid ratio.