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目的 观察水杨酸镁对脑细胞内钙的影响 ,探讨其中枢神经系统的钙拮抗作用。方法 应用新一代钙离子指示剂Fura 2 /AM技术 ,用双波长荧光分光光度计测定细胞内钙浓度。结果 在不同外钙条件下 (外钙 0 ,0 0 1,0 1,1 0 ,1 3mmol·L-1)细胞内静息钙浓度分别 41± 5 ,5 9± 6 ,84 7± 2 5 ,10 4± 7,(12 6± 5 )nmol·L-1(各组n =8)。水杨酸镁0 2mmol·L-1对静息细胞内钙无显著影响 (P >0 0 5 )。但水杨酸镁 (0 0 5～ 0 4mmol·L-1)可以剂量依赖性抑制高钾及L 谷氨酸诱发的细胞内钙增高。其IC50 分别为 0 32 3mmol·L-1(95 %CI为 0 12 2～ 0 85 4mmol·L-1)和 0 12 4mmol·L-1(95 %CI为 0 0 73～ 0 2 10mmol·L-1)。结论 水杨酸镁通过抑制电压依从性及受体操纵型的钙通道 ,对中枢神经系统具有较强的钙拮抗作用。
Objective To observe the effect of magnesium salicylate on intracellular calcium and to explore its calcium antagonism in the central nervous system. Methods A new generation of calcium ion indicator Fura 2 / AM was used to measure intracellular calcium concentration by dual wavelength fluorescence spectrophotometer. Results The intracellular calcium concentrations were 41 ± 5, 59 ± 6 and 84 7 ± 2 5, respectively, under different exogenous calcium concentrations (0,01,0,1,1,0 and 13 mmol·L-1) , 10 4 ± 7, (12 6 ± 5) nmol·L-1 (n = 8 in each group). Salicylic acid 0 2 mmol·L-1 had no significant effect on resting intracellular calcium (P> 0.05). However, magnesium salicylate (0 0 5 ~ 0 4 mmol·L-1) could inhibit the increase of intracellular calcium induced by high potassium and L-glutamate in a dose-dependent manner. The IC50 values were 0 32 3 mmol·L -1 (95% CI 012 2-0 85 4 mmol·L -1) and 0 12 4 mmol·L -1 (95% CI 0 073-0 102 mmol·L -1, respectively) -1). Conclusions Magnesium salicylate has strong calcium antagonism on the central nervous system by inhibiting voltage compliance and receptor-operated calcium channels.