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目的:探讨TLR-4介导的免疫逃逸信号转导系统在膀胱尿路上皮癌免疫逃逸机制中的作用及其与膀胱癌生物学行为的关系。方法:采用免疫组化染色法检测60例膀胱尿路上皮癌和10例正常膀胱组织中TLR-4、B7-H1、PD-1、Fas及FasL的表达。结果:TLR-4在正常膀胱组织中均有表达,而在膀胱尿路上皮癌中表达有所降低;B7-H1、PD-1、Fas、FasL在正常膀胱组织中无表达或表达极低,而在膀胱尿路上皮癌中的表达均明显增高,并且其表达与肿瘤的病理分级和临床分期密切相关,同时B7-H1表达与复发亦密切相关。结论:TLR-4介导的免疫逃逸信号转导系统可能与膀胱尿路上皮癌的免疫逃逸密切相关,在膀胱尿路上皮癌的发生发展中可能存在着TLR-4介导的免疫逃逸信号转导系统的活化和相关免疫抑制分子表达的上调。
Objective: To investigate the role of TLR-4-mediated immune escape signal transduction system in the immune escape mechanism of bladder urothelial carcinoma and its relationship with the biological behavior of bladder cancer. Methods: The expressions of TLR-4, B7-H1, PD-1, Fas and FasL in 60 cases of bladder urothelial carcinoma and 10 cases of normal bladder tissue were detected by immunohistochemical staining. Results: The expression of TLR-4 in normal bladder tissues and the expression of TLR-4 in bladder urothelial carcinoma tissues were lower than those in normal bladder tissues. The expressions of B7-H1, PD-1, Fas, However, the expression of B7-H1 in bladder urothelial carcinoma was significantly increased, and its expression was closely related to the pathological grade and clinical stage of the tumor. Meanwhile, the expression of B7-H1 was also closely related to the recurrence. CONCLUSION: TLR-4-mediated immune escape signal transduction system may be closely related to the immune escape of bladder urothelial carcinoma. TLR-4-mediated immune escape signal may exist in the development of bladder urothelial carcinoma Activation of the lead system and up-regulation of related immunosuppressive molecule expression.