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以提纯的ＣＭＶ作为抗原，经静脉注射、肌肉注射和足垫注射等对家兔进行免疫，获得高效价的抗血清（琼脂双扩散效价为１／２５６）；在间接ＥＬＩＳＡ试验过程中，用ＰＢＳ＋０．０５％ＴｒｉｔｏｎＸ－１００作为样本研磨液，用１：１６０００稀释度的ＣＭＶ抗血清或Ｚμｇ／ｍｌＣＭＶ抗血清的ＩｇＧ作为第一抗体，用１：１０００稀释度的冻干辣根过氧化物酶标记的羊抗兔的ＩｇＧ作为第二抗体，以待测样本光密度值与阴性对照光密度值之比大于或等于３作为判断待测样本为阳性反应的标准，结果表明：间接ＥＬＩＳＡ试验的灵敏度比生物检测的灵敏度要高３２～１６０倍。用间接ＥＬＩＳＡ检测矮牵牛的病毒样本３０份，ＣＭＶ占７０％。
Purified CMV was used as antigen to immunize rabbits by intravenous injection, intramuscular injection and footpad injection to obtain high titer antiserum (agar double diffusion titer was 1/256). In the indirect ELISA test, PBS + 0.05% Triton X-100 as a sample polishing solution and IgG as a primary antibody at a 1: 16000 dilution of CMV antiserum or Z g / ml of CMV antiserum, using a 1: 1000 dilution of lyophilized horseradish peroxidase Labeled goat anti-rabbit IgG as the second antibody, the ratio of the optical density of the sample to the negative control optical density value is greater than or equal to 3 as the standard for judging the positive reaction of the sample to be tested. The results show that the sensitivity of the indirect ELISA test Than the biological detection sensitivity 32 to 160 times higher. 30 samples of petunia virus were detected by indirect ELISA, accounting for 70% of CMV.