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本实验采用Wachstein-Meisel显示ATP酶的方法,对小白鼠艾氏腹水癌细胞的Mg~(++)-ATP酶进行光学及电子显微镜下的细胞化学观察,见到Mg~(++)-ATP酶集中分布在癌细胞的质膜表面和线粒体内,线粒体内存在的Mg~(++)-ATP酶对戊二醛极为敏感,一经戊二醛固定后,线粒体内存在的Mg~(++)-ATP酶反应消失。但质膜上Mg~(++)-ATP酶的沉淀反应却不受戊二醛的影响,固定后仍可见到强的沉淀反应。L-半胱氨酸可加强质膜上和线粒体内ATP活性酶所产生的阳性反应,而经NaF,PCMB处理后,其阳性的沉淀反应消失。
In this experiment, the Wachstein-Meisel ATPase method was used to observe the Mg~(++)-ATPase of Ehrlich ascites carcinoma cells in mice and the cytochemical observation under the electron microscope. Mg~(++)- was seen. ATPase is concentrated on the surface of plasma membrane and mitochondria of cancer cells. Mg ++ ATPase present in mitochondria is extremely sensitive to glutaraldehyde. Once glutaraldehyde is fixed, mitochondrial Mg~(+) is present. +) - The ATPase reaction disappears. However, the precipitation reaction of Mg ++ ATPase on the plasma membrane was not affected by glutaraldehyde, and a strong precipitation reaction was still observed after fixation. L-cysteine can enhance the positive reaction produced by ATP-active enzymes on the plasma membrane and in mitochondria, but after NaF and PCMB treatment, its positive precipitation reaction disappears.