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本研究利用~(60)Co γ射线辐照亚麻下胚轴愈伤组织,诱导培养再生植株,结果表明:低于2.0KR 辐照对亚麻愈伤组织培养出苗所需要的时间无明显影响;辐射在0.5KR 时,对愈伤组织分化出苗有明显的促进效应;辐照在1.0KR 时,个别品种愈伤组织分化率和绿苗繁殖频率仍明显高于对照;照射量达到3.0KR 时,愈伤组织出苗时间延长,愈伤组织分化率和绿苗繁殖频率与对照相比明显降低;当照射量达到5.0KR 时,个别品种愈伤组织已不能出苗,有的品种虽然可以出苗,但多为畸形苗,在壮苗过程中难以存活,已无试验价值。
In this study, γ-rays irradiated with ~ (60) Co γ-rays were used to induce the hypocotyls of flax, and the regenerated plants were induced to induce regeneration. The results showed that irradiation with less than 2.0KR had no significant effect on the time required for seedlings to emerge. At 0.5KR, the differentiation of callus had obvious promotion effect. At the 1.0KR irradiation, the differentiation rate of callus and the frequency of green seedlings were still significantly higher than that of the control. When the irradiation reached 3.0KR, Compared with the control, the frequency of callus differentiation and the rate of callus differentiation decreased obviously when the rate of emergence was 5.0KR. However, the callus of some varieties could not emerge when the irradiation dose reached 5.0KR. Malformation seedlings, in the process of strong seedling difficult to survive, no test value.