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目的:从升麻中分离得到升麻苷H-1并建立其定量测定方法。方法:采用硅胶柱色谱从升麻乙醇提取液的水不溶性部位分离得到升麻苷H-1;采用HPLC法,色谱柱:Hypersil BDS C18柱(200mm×4.6mm,5μm),流动相:甲醇-水(60∶40),检测波长:210nm,流速:1.0ml/min,柱温:30℃。结果:升麻苷-1在0.358~6.444mg范围内呈现良好的线性关系(r=0.9996),精密度、稳定性和重现性的RSD均小于2%,平均加样回收率为101.9%,RSD为4.81%。结论:该方法准确、快速、重现性好,可用于测定升麻药材中升麻苷H-1的含量。
OBJECTIVE: To isolate and characterize cimicroside H-1 from cimicifuga and to establish a method for its quantitative determination. Methods: The chromatographic separation was performed on a Hypersil BDS C18 column (200mm × 4.6mm, 5μm) using HPLC. The mobile phase consisted of methanol - Water (60:40), detection wavelength: 210 nm, flow rate: 1.0 ml / min, column temperature: 30 ° C. Results: The calibration curve showed a good linearity (r = 0.9996) in the range of 0.358 ~ 6.444mg. The RSDs of precision, stability and reproducibility were all less than 2%. The average recoveries were 101.9% RSD is 4.81%. Conclusion: The method is accurate, rapid and reproducible. It can be used to determine the content of cortisol H-1 in the Radix Astragali.