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目的初步探讨缺氧时Pept1、Oatp1b1、Oat1、Oct1、Mdr1和Mrp2 6种药物转运体基因表达的变化。方法Wistar大鼠随机分为正常对照组、缺氧24h和72h组,缺氧组置于模拟海拔5000m(压力:397.5mmHg,氧分压:42mmHg)的低压氧舱中。提取各组大鼠小肠、肝脏、肾脏组织中的总RNA,以Real-time PCR方法检测6种药物转运体基因表达量。结果与正常对照组相比,缺氧组中6种药物转运体基因表达大多数升高,但随着时间的延长,不同组织中的不同转运体出现不一样的变化趋势。结论高原缺氧会影响药物转运体mRNA的表达水平,这可为高原药物转运体及药代动力学研究提供一定的理论依据。
Objective To investigate the gene expression changes of six kinds of drug transporters Pept1, Oatp1b1, Oat1, Oct1, Mdr1 and Mrp2 during hypoxia. Methods Wistar rats were randomly divided into normal control group, hypoxia 24h and 72h group, hypoxia group was placed in the hypoxic chamber of simulated altitude 5000m (pressure: 397.5mmHg, oxygen partial pressure: 42mmHg). The total RNA was extracted from the small intestine, liver and kidney of each group, and the expression of six kinds of drug transporter genes was detected by Real-time PCR. Results Compared with the normal control group, the expression of six kinds of drug transporter genes in hypoxia group were mostly increased. However, with the extension of time, different transporters in different tissues showed different trends. Conclusion High altitude hypoxia can affect the expression of drug transporter mRNA, which may provide some theoretical basis for the study of plateau drug transporter and pharmacokinetics.