Objective:To screen the antimicrobial potential of three ethnomedicinal plants Chassalia curviflora Thw.(C.curviflora),Cyclea peltata Hook.F.& Thomson(C.peltata) and Euphorbia hirta L(E.hirta) used in folk medicines in Aarukani hills Kani tribe,Tamil Nadu,India against human bacterial pathogens.Methods:Antibacterial efficacy was performed by disc diffusion method against the pathogens viz.,Escherichia coli(E.coli)(ATCC 35218),Staphylococcus aureus (S.aureus)(ATCC 6538),Salmonella typhi(S.typhi)(MTCC 733),Proteus vulgaris(P.vulgaris), Proteus mirabilis(P.mirabilis) and Streptococcus pyogenes(S.pyogenes) and incubated for 24 h at 37°C.Results:The maximum degree of antibacterial activity was observed in C.peltata followed by C.curviflora.While E.hirta showed comparatively low degree of antibacterial activity.The methanolic extract of C.peltata showed the antibacterial activity against three pathogens viz.,S.pyogenes,P.vulgaris and E.coli with the inhibition zones 12 mm,10 mm and 9 mm,respectively,hexane extracts of C.peltata also showed the antibacterial activity against two selected pathogens viz.,P.vulgaris and P.mirabilis with 15 mm and 12 mm of inhibition zones.All the three different concentrations(025,0.50 & 0.75 mg/mL) of methanolic extract of C. peltata show the inhibitory effect on the three susceptible bacteria S.pyogenes,P.vulgaris and E. coli with the maximum inhibition in the highest concentration(0.75 mg/mL).The methanolic and hexane extracts of C.curviflora exhibited the antibacterial activity against only one bacterium each i.e.P.vulgaris and S.typhi with the maximum zone of inhibition 13 and 11 mm respectively. The methanolic and hexane extracts of E.hirta exhibited the antibacterial activity against only one bacterium Le.S.pyogenes with the maximum zone of inhibition 13 and 11 mm respectively. Conclusions:The present investigation revealed that the C.curviflora,C.peltata and E.hirta are potentially good source of antibacterial agents and demonstrates the importance of such plants in traditional medicines. Objective: To screen the antimicrobial potential of three ethnomedicinal plants Chassalia curviflora Thw. (C. curviflora), Cyclea peltata Hook. F. & Thomson (C.peltata) and Euphorbia hirta L (E.hirta) used in folk medicines in Aarukani hills Kani tribe, Tamil Nadu, India against human bacterial pathogens. Methods: Antibacterial efficacy was performed by disc diffusion method against the pathogens viz., Escherichia coli (ATCC 35218), Staphylococcus aureus (S. aureus) (ATCC 6538 ), Salmonella typhi (S. typhi) (MTCC 733), Proteus vulgaris (P. vulgaris), Proteus mirabilis (P. mirabilis) and Streptococcus pyogenes (S. pyogenes) and incubated for 24 h at 37 ° C. Results: The maximum degree of antibacterial activity was observed in C.peltata followed by C. curviflora. Whilst E. hirta showed comparatively low degree of antibacterial activity. methanolic extract of C.peltata showed the antibacterial activity against three pathogens viz., S. pyogenes, P. vulgaris and E. coli with the inhibition zones 12 mm, 10 mm and 9 mm, res pectively, hexane extracts of C.peltata also showed the antibacterial activity against two selected pathogens viz., P. vulgaris and P. mirabilis with 15 mm and 12 mm of inhibition zones. All the three different concentrations (025,0.50 & 0.75 mg / mL) of methanolic extract of C. peltata show the inhibitory effect on the three susceptible bacteria S. pyogenes, P. vulgaris and E. coli with the maximum inhibition in the highest concentration (0.75 mg / mL). methanolic and hexane extracts of C.curviflora exhibited the antibacterial activity against only one bacterium each iePvulgaris and S.typhi with the maximum zone of inhibition 13 and 11 mm respectively. The methanolic and hexane extracts of E.hirta exhibited the antibacterial activity against only one bacterium Le.S Conclusions: The present investigation revealed that the C.curviflora, C.peltata and E.hirta are potentially good source of antibacterial agents and demonstratesthe importance of such plants in traditional medicines.