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目的探讨我国褪黑色素受体1B(MTNR1B)基因SNP位点rs10830963和rs1387153的多态性与妊娠期糖尿病(GDM)遗传易感性的关系。方法采用病例对照研究,分别选取GDM患者(GDM组)184例和对照(NC)组235名,利用PCR-RFLP的方法测定2个SNP位点多态性分布,并进行统计学分析。结果 GDM组FPG高于NC组(P=0.039),但孕前BMI比较差异无统计学意义。SNP位点rs10830963基因型(GG,GC,CC)频率、等位基因频率与NC组比较,差异均无统计学意义(P=0.637,P=0.422)。而SNP位点rs1387153基因型(TT,CT,CC)与NC组比较,差异有统计学意义(P=0.012),且风险基因型TT的频率高于NC组(TTvs TC+CC,P=0.01)。GDM组T等位基因高于NC组(OR:1.517,95%CI:1.147~2.006,P=0.003),且该SNP位点的TT基因型的GDM患者FPG高于其他两个基因型的患者。结论 MTNR1B基因SNP位点rs10830963与GDM无相关性,而SNP位点rs1387153与GDM的易感性相关。
Objective To investigate the relationship between genetic polymorphisms of SNP rs10830963 and rs1387153 of melatonin receptor 1B (MTNR1B) gene and genetic susceptibility to gestational diabetes mellitus (GDM) in China. Methods A case-control study was conducted in which 184 cases of GDM patients (GDM group) and 235 cases of control group (NC) were enrolled. The polymorphism distributions of two SNPs were detected by PCR-RFLP and analyzed statistically. Results FPG in GDM group was higher than that in NC group (P = 0.039), but there was no significant difference in BMI before pregnancy. SNP rs10830963 genotype (GG, GC, CC) frequency, allele frequency compared with the NC group, the difference was not statistically significant (P = 0.637, P = 0.422). The SNP rs1387153 genotypes (TT, CT, CC) were significantly different from those in NC group (P = 0.012), and the risk genotype TT frequency was higher than that in NC group (TTvs TC + CC, P = ). The T allele of GDM group was higher than that of NC group (OR: 1.517, 95% CI: 1.147-2.006, P = 0.003), and the FPG of TT genotype of GDM group was higher than that of the other two genotypes . Conclusion MTNR1B gene SNP locus rs10830963 and GDM no correlation, and SNP locus rs1387153 and GDM susceptibility.