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目的:研究薯蓣皂苷元对体外培养的大鼠成骨细胞增殖、分化及护骨素/核因子κB受体活化因子配基(OPG/RANKL) mRNA的影响,探讨薯蓣皂苷元预防与治疗骨质疏松的作用机制。方法:在体外培养的大鼠成骨细胞培养基中分别加入不同浓度的薯蓣皂苷元作用不同时间,用MTT法检测药物对成骨细胞增殖的影响,检测成骨细胞内ALP活性分析药物对成骨细胞分化的影响,用半定量RT-PCR检测成骨细胞内OPG/RANKL mRNA的表达量,分析药物对破骨细胞重要信号传导通路OPG/RANKL/RANK系统的影响。结果:3.64×10-8-3.64×10-7mol/L的薯蓣皂苷元作用于大鼠成骨细胞3d,可促进成骨细胞的增殖(P<0.01),上调成骨细胞内OPG/RANKL mRNA的比值(P<0.01);作用7d,可提高成骨细胞内ALP活性(P<0.05或P<0.01)。结论:适量浓度薯蓣皂苷元可促进成骨细胞的增殖、分化及抑制破骨细胞的形成。
Objective: To investigate the effects of diosgenin on the proliferation and differentiation of rat osteoblasts cultured in vitro and the effect of OPG / RANKL mRNA on the expression of OPG / RANKL mRNA in cultured rat osteoblasts. Loose mechanism of action. Methods: Different concentrations of diosgenin were added into rat osteoblast culture medium in vitro for different time. The effect of drugs on the proliferation of osteoblasts was detected by MTT assay. The effect of ALP activity assay on osteoblasts The expression of OPG / RANKL mRNA in osteoblasts was detected by semi-quantitative RT-PCR and the effect of drugs on the OPG / RANKL / RANK system of osteoclasts was analyzed. Results: The diosgenin of 3.64 × 10-8-3.64 × 10-7mol / L could promote the proliferation of osteoblasts (P <0.01) and up-regulate the expression of OPG / RANKL mRNA (P <0.01). After treated for 7 days, ALP activity in osteoblasts increased (P <0.05 or P <0.01). Conclusion: Appropriate concentration of diosgenin can promote the proliferation and differentiation of osteoblasts and inhibit the formation of osteoclasts.