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1976年血液学国际标准化委员会(以下简称ICSH)成立了一个染色和染色方法专家小组。该小组负责提出血和骨髓片罗曼诺夫斯基染色法的标准化方法。小组成员几次会晤,同时开展各实验室之间的协作研究。自1979年以来,他们分别发表了不少文章,证实了天青E和曙红Y两种染料能充分显示罗曼诺夫斯基染色的特征及影响染色的各种因素。本文叙述的“ICSH”标准化建议是根据专家小组和其他同行专家们(包括欧洲经济委员会参比局专家组)所做工作。罗曼诺夫斯基染色对造血细胞和其他种类细胞的形态学鉴定非常重要。
1976 Hematology International Standardization Committee (hereinafter referred to as ICSH) set up a staining and staining expert group. The team is responsible for proposing a standardized method for the Romanovsky staining of blood and bone marrow pieces. The team members met several times and conducted collaborative research among the laboratories. Since 1979, they published a lot of articles respectively, confirming the two dyes of Azure E and Eosin Y can fully display the characteristics of the Romanovsky staining and various factors that affect the dyeing. The “ICSH” standardization proposal described in this article is based on work done by expert groups and other peer experts, including the expert group of the Bureau of Reference of the Economic Commission for Europe. Romanovsky staining is very important for the morphological characterization of hematopoietic cells and other kinds of cells.