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目的 :探讨p5 3基因单链构象多态性变化与食管鳞癌患者术后生存期的关系。方法 :采用聚合酶链反应 单链构象多态性 (PCR SSCP)结合银染技术 ,对 47例河南林县食管鳞癌患者癌组织及癌旁正常组织中p5 3基因第 5～ 8外显子进行比较。结果 :p5 3基因第 5～ 8外显子出现异常电泳条带者 2 0例 (4 2 6 % ) ;生存期大于 5a组与生存期小于 5a组 ,p5 3基因单链构象多态性变化发生率分别为 32 1% (9/ 2 8)和 5 7 9(11/ 19) ,2组比较 ,差异无显著性(χ2 =3 0 71,P >0 0 5 )。结论 :食管鳞癌组织p5 3基因第 5～ 8外显子单链构象多态性变化与患者生存期无明显相关关系
Objective: To investigate the relationship between single-stranded conformation polymorphism of p5 3 gene and survival time of esophageal squamous cell carcinoma patients. Methods: PCR-SSCP and silver staining were used to detect the expression of exon 5 ~ 8 of p5 3 gene in 47 patients with esophageal squamous cell carcinoma of Linxian, Henan Province and adjacent normal tissues Compare. Results: There were 20 cases (42.6%) of abnormal electrophoresis bands in exon 5 to exon 8 of p5 3 gene, the single strand conformation polymorphism of p5 3 gene in survival time longer than 5a group and survival time less than 5a group The incidence rates were 32 1% (9/2 8) and 5 7 9 (11/19) respectively. There was no significant difference between the two groups (χ2 = 3 0 71, P 0 05). Conclusion: The single-strand conformation polymorphism of exon 5-8 of esophageal squamous cell carcinoma has no significant correlation with the survival of patients