Lentivirus相关论文
Within less than a decade since its inception,CRISPR-Cas9-based genome editing has been rapidly advanced to human clinic......
Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient
Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males.Thus,SSCs are a potential tar......
...
Lentivirus transduced interleukin-1 receptor antagonist gene expression in murine bone marrow-derive
Genetically modified mesenchymal stem cells have been used in attempts to increase the expression of interleukin-1 recep......
The effects of biomimetic nano-hydroxyapatite/polyamide66 composite scaffolds on the growth of LvNel
Nel-like molecule-1(Nell-1)is a novel osteogenic growth factor that has been found to specifically stimulate osteogenic ......
PRIM1 acts on the DNA replication initiation site to regulate proliferation and apoptosis of hepatoc
目的 To explore the role of PRIM1 in liver cancer 方法 Quantitative polymerase chain reaction(QPCR)was used to detect mR......
Construction of a Lentivirus Vector Containing shRNA Targeting ADAM17 and it Role in Attenuating End
Objective: Systemic inflammatory response syndrome(SIRS)is a pathophysiological inflammatory response mediated largely b......
...
目的:探索Lentivirus介导分泌神经营养因子-3(NT-3)直接体内转基因治疗人鼠脊髓损伤机理和治疗作用。方法:将28只Wistar大鼠在T10水......
目的:以Lentivirus为载体,构建同时表达GFP和NT-3的鼠胚基因工程NSC,以便为脊髓损伤研究提供材料。方法:①培养和鉴定胎鼠神经干细胞(N......
目的:探索以Lentivirus为载体,构建同时表达绿色荧光蛋白(greenfluorescence protein,GFP)和神经营养因子-3(neurotrophicfactor-3,NT-......
神经干细胞(NSC)是中枢神经系统中具有自我更新能力和多种分化满能的细胞,是脊髓损伤(SCI)后再生修复的理想材料和基因载体。本文探......
目的:探索以Lentivirus(慢病毒)为载体,构建同时携带并表达多基因的基因工程人胚神经干细胞(HumanNeuralStemCell,hNSC)的可行性,以......
会议
The experimental study of genetic engineering human neural stem cells mediated by lentivirus to expr
...
Esophageal cancer,including esophageal squamous cell carcinoma and esophageal adenocarcinoma,is one of the leading c......
Downregulation of SNAIL sensitizes hepatocellular carcinoma cells to TRAIL-induced apoptosis by regu
Hepatocellular carcinoma (HCC) is the sixth most common cancer and the second most lethal cancer worldwide.Evidence ......
目的 研究慢病毒介导生存素基因(Lentivirus-Survivin)对早、晚期反分化人椎间盘髓核细胞的生物学效应。方法应用组织块法分离培养人......
慢病毒介导的RNAi具有转移基因效率高,作用持久稳定等特点,成为基因治疗和基因功能研究的重要工具。本试验中将在细胞水平验证可以......
建立HPV16-E7基因的RNAi细胞模型,可为进一步研究HPV16-E7蛋白作用及致癌机制提供物质前提。选择HPV16-E7基因特异的siRNA片段,构建......
目的观察慢病毒介导的神经元特异性生长抑素(somatostatin,SST)过表达对大鼠海马电点燃癫痫的抑制作用。方法成年Sprague-Dawley (S......
Survival of transplanted neurotrophin-3 expressing human neural stem cells and motor function in a r
...
Lentiviral-mediated vascular endothelial growth factor 165 gene transfer into neural stem cells prom
We constructed a lentiviral vector carrying vascular endothelial growth factor 165,which was used to transfect neural st......
Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchy
microRNAs (miRNAs) play an important regulatory role in the self-renewal and differentiation of stem cells. In this stud......
...
...
目的 构建醌型二氢生物碟呤还原酶(quinonoid dihydropteridine reductase,QDPR)敲低慢病毒模型,检测其对肾小管上皮NRK-52E细胞QD......
构建1株能够稳定表达FABP4的小尾寒羊成纤维细胞系,用于小尾寒羊未来的品种保护、开发与利用,首先利用组织块培养法制备了小尾寒羊......
目的构建人miR-146b慢病毒表达载体,包被病毒并检测其在人脂肪细胞中过表达效果。方法以人基因组DNA为模板,PCR高保真扩增包含miR-......
目的 建立稳定抑制β-eatenin基因表达的入神经母细胞瘤BE2C细胞株,为探讨Wnt/-eatenin信号在神经母细胞瘤发生中的作用提供细胞模......
随着分子生物学和基因工程学的进展,为了弥补电子起搏器的不足,生物起搏器应运而生.基因治疗作为构建生物起搏器的一个切实可行的......
目的探讨慢病毒介导的热休克蛋白70(HSP70)表达对缺血/缺氧神经细胞细胞膜钙离子通道的影响及其机制。方法取对数生长期的嗜铬细胞瘤......
目的 探讨小分子RNA干扰雷帕霉素靶蛋白(mTOR)基因表达对百草枯致大鼠肺纤维化的影响.方法 体外培养人胚肾细胞HEK-293,构建mTOR小......
Lentivirus mediated shRNA interference targeting MAT2B induces growth-inhibition and apoptosis in he
AIM: To investigate the effects of lentivirus vector mediated short hairpin RNA interference targeting methionine adenos......
In order to explore the role of TNF-a in Niemann-Pick type C (NPC) disease,lentivi-ral-delivered RNA interference (RNAi)......
PPM1D Silencing by Lentiviral-mediated RNA Interference Inhibits Proliferation and Invasion of Human
To construct a lentiviral shRNA vector targeting human protein phosphatase 1D magnesium-dependent (PPM1D) gene and detec......
目的:探索以Lentivirus为载体,构建同时携带并表达多基因的基因工程人胚神经干细胞(human neural stem cell,hNSC)的可行性,为脊髓......
目的 构建小鼠miR-29c过表达重组慢病毒载体,并对表达产物进行鉴定.方法 应用PCR法从小鼠MLE-12细胞中提取pre-miR-29c基因,测序后......
Objective: The aim of the study was to investigate the interference and anti-tumor effects of lentiviral vector of miRNA......
目的:利用慢病毒表达载体将HIV-1Vif基因导入原发性渗出性淋巴瘤(PEL)细胞BCBL-1中使之持续表达目的蛋白Vif,并检测Vif对其中潜伏K......
目的:构建人类疱疹病毒6型U94基因慢病毒载体,研究U94基因对血管内皮细胞增殖及血管生成的影响。方法:以质粒pSR2PH-U94为模板,PCR......
目的:构建含活性缺陷型小鼠端粒酶催化亚基(去除氨基酸702~712的mTERT,命名为mTERT△)基因的慢病毒载体,检测其表达和功能.方法:从......
目的:构建和鉴定大鼠肝细胞生长因子(HGF)shRNA慢病毒载体,为在分子水平进一步研究HGF对巨核细胞成熟与分化能力的影响提供方法。......
目的:探讨β3肾上腺素能受体(β3-AR)对SD大鼠乳鼠心肌肥大的影响及其机制。方法:体外培养SD大鼠乳鼠心肌细胞,用携带β3-AR基因的......
目的 构建RNA干扰整合素连接激酶(ILK)基因的慢病毒载体并转染胰腺癌细胞(Panc-1),并验证其干扰有效性.方法 对胰腺癌Panc-1细胞进......
目的 研究基因沉默ILK对胰腺癌细胞 (Panc-1) 增殖能力的影响.方法 对胰腺癌Panc-1细胞进行细胞培养, 成功构建ILK-specific shRNA......
目的构建HJURP基因的RNA干扰慢病毒表达载体并在人胚胎绒毛细胞上鉴定其沉默效率,进而提供HJURP基因缺陷表达的原代胚胎绒毛细胞模......
目的 在体外环境下同时下调细胞中Noggin基因和增加BMP-14基因,观察对脂肪来源干细胞(adipose derived stem cells,ADSCs)成骨分化......