DEVELOPMENT OF MULTIPLEX PCR FORDETECTION OF B.cereus, S.aureus, AND E.coli AND ITS USAGE IN THE LEF

来源 :第十四届世界食品科技大会 | 被引量 : 0次 | 上传用户：morpheus

【摘要】

：

In order to investigate the possible foodbome pathogens hn ready-to-eat food such as cooked and leftover rice,a simple and rapid multiplex-PCR protocol for detection of Bacillus cereus,Staphylococcus

【作者】

：

XiuLan Li

【机构】

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Food Safety Technology Unit(FSTU), Department of Food & Engineering, Jinan University, Guangzhou, Ch

【出处】

：

第十四届世界食品科技大会

【发表日期】

：

2008年1期

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　　In order to investigate the possible foodbome pathogens hn ready-to-eat food such as cooked and leftover rice,a simple and rapid multiplex-PCR protocol for detection of Bacillus cereus,Staphylococcus aureus and Escherichia coli wasdeveloped.Three primer sets which were specific to B.cereus (Bal),S.aureus (Sa0856) and E.coli (uspA) respectively and one universal primer (16S rRNA primer,used as a internal control) were combined into a primer pool.

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DEVELOPMENT OF MULTIPLEX PCR FORDETECTION OF B.cereus, S.aureus, AND E.coli AND ITS USAGE IN THE LEF

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