【摘 要】
：
目的：探索应用长爪沙鼠(Meiiones Unguiculataus Milme-Edwauds)构建HEV动物模型的可行性。方法：本实验从猪粪便稀释悬液中提取HEV RNA并鉴定，经腹腔和口服接种HEV于长爪沙鼠，并每周采集血液、相关器官(心、肝、脾、肺、肾、肠系膜淋巴结、脾脏)及粪便。用RT-N-PCR和免疫组化等检测方法分析各个样品。
【机 构】
：
军事医学科学院军事兽医研究所,长春,130122 军事医学科学院军事兽医研究所,长春,130122
【出 处】
：
第十一届全国病毒学学术研讨会暨第六届武汉现代病毒学国际研讨会
论文部分内容阅读
目的：探索应用长爪沙鼠(Meiiones Unguiculataus Milme-Edwauds)构建HEV动物模型的可行性。方法：本实验从猪粪便稀释悬液中提取HEV RNA并鉴定，经腹腔和口服接种HEV于长爪沙鼠，并每周采集血液、相关器官(心、肝、脾、肺、肾、肠系膜淋巴结、脾脏)及粪便。用RT-N-PCR和免疫组化等检测方法分析各个样品。
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