Therapeutic strategies based on the modulation of microRNA activity possess much promise in cancer gene therapy.MieroRNA-34a (miR-34a) is a mieroRNA regulated by the p53 network at transcriptional level and has been demonstrated to be remarkably down-regulated in a variety of cancers [1].Exogenous expression of miR-34a could induce cell apoptosis and inhibit cell proliferation and migration targeting Notch pathway and Bcl-2.To increase the delivery efficiency of miR-34a,it is necessary to construct targeting delivery systems.Chondroitin sulfate (CS) is a natural polysaccharide with high affinity to CD44 which is over-expressed in many solid tumors [2],and thus CS could be used as a specific ligand targeting cancer cells via CD44-mediated endocytosis.