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[Objective]: To analyze the effect of boiling treatment on the allergenicity changes of tropomyosin (TM) in aquatic products,Pacific white shrimp (Litopenaeus vannamei),black tiger shrimp (Penaeus monodon),Chinese Mitten Crab (eriocheir sinensis),short necked clam (Venerupis philippinarum) and razor clam (Sinonovacula constrzcta) were choosed as samples.[Methods]:We chose SDS-PAGE and ELISA technologies to compare the allergenicity changes of TM after boiling treatment.