Objectives Barretts esophagus (BE) is recognized as a complication of chronic gastroesophageal acid and bile reflux, and also considered to be a precancerous state in the esophagus and may progress to esophageal adenocarcinoma (EA).H.pylori has been found to colonize the Barrett epithelium of lower esophagus.However, its role in the development of Barretts esophagus and esophageal adenocarcinoma is unclear.Here, we explored the effects of acidic deoxycholic acid and H.pylori on esophageal cell lines in vitro, with a particular focus on whether NF-kB is involved in this event.