【摘 要】
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Purpose: To demonstrate the effects of a novel Smac/DIABLO mimetic APG1387 on properties of nasopharyngeal carcinoma cancer stem-like cells, and invest further to establish the mechanisms thereof.Meth
【机 构】
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中山大学肿瘤防治中心,华南肿瘤重点实验室,广东广州,510060
【出 处】
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2015医学前沿论坛暨第十四届全国肿瘤药理与化疗学术会议
论文部分内容阅读
Purpose: To demonstrate the effects of a novel Smac/DIABLO mimetic APG1387 on properties of nasopharyngeal carcinoma cancer stem-like cells, and invest further to establish the mechanisms thereof.Method: FACS was used to sort SP cells.Suspension culture assay was performed to detect nasosphere.Western-blotting was used to detect the protein levels.Immunoprecipitation was employed to determine the protein-protein interactions.Molecular cloning and lipofectamine transfection technology was used for plasmid construction, transfection and RNA Interference.Results: We found that the S18 cell line had higher SP population portion and stronger ability of sphere-formation and metastasis, which suggests higher CSC-like properties than other NPC cell lines.
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