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Background: The prevalence of P[4] group A rotaviruses(RVAs)circulating in children with acute diarrhea in Guangxi Province,China in 2014 increased significantly compared to that in 2013,while the combined VP7 genes could not be typed.Method: Further molecular analyses for the VP7 gene were conducted by RT-PCR with modified primers through exhausted searching and blasting of G2 VP7 gene sequences and sequencing.