论文部分内容阅读
目的 观察蜂毒素基因重组腺病毒诱导肝癌细胞凋亡的作用。 方法 通过形态学观察、DNA电泳、原位缺口末端标记(TUNEL)、流式细胞仪等方法观察携蜂毒素基因重组腺病毒对肝癌细胞凋亡的影响。 结果 光镜下见部分肝癌细胞出现凋亡形态学变化,DNA电泳可见梯形条带,TUNEL染色结果显示,Ad-rAFP-Mel组、Ad-rAFP组和对照组的凋亡率分别为(21.5±2.4)％、(10.5±4.4)％和(3.0±1.4)％,各组间差异有显著性(F=3 8.0,P<0.0 5)。流式细胞仪定量分析结果显示,3组凋亡率分别为(7.3±0.5)％、(3.9±0.1)％和(0.8±0.1)％,各组间差异有显著性(F=415.1,P<0.05)。 结论 诱导细胞凋亡可能是蜂毒素抗肿瘤的作用机制之一。
Objective To observe the role of melittin gene recombinant adenovirus in inducing apoptosis of hepatocellular carcinoma cells. Methods Morphological observation, DNA electrophoresis, TUNEL and flow cytometry were used to observe the effect of recombinant adenovirus carrying melittin on the apoptosis of hepatocellular carcinoma cells. Results The morphology of apoptotic cells in some hepatoma cells was observed by light microscopy. Trapezoidal bands were observed by DNA electrophoresis. The apoptotic rates of Ad-rAFP-Mel group, Ad-rAFP group and control group were (21.5 ± 2.4)%, (10.5 ± 4.4)% and (3.0 ± 1.4)%, respectively. There was significant difference among the groups (F = 3 8.0, P <0.05). The results of flow cytometry showed that the apoptotic rates in the three groups were (7.3 ± 0.5)%, (3.9 ± 0.1)% and (0.8 ± 0.1)%, respectively, with significant differences among the groups (F = 415.1, P <0.05). Conclusion Induction of apoptosis may be one of the mechanisms of antitumor effect of melittin.