论文部分内容阅读
Cholangiocarcinoma(CCA) is one of the highest malignant tumors of digestive system which was coming from bile duct epithelium. CCA occurs with a higher incidence in our country. The prognosis is poor, because of the unavailability of early diagnosis and against chemotherapy. The cause of cholangiocarcinoma is related to the disequilibrium of cytokines. In the present study, the relations between the CCA and cytokines are getting more and more attentions. TNF (tumor necrosis factor) is a very important kind of cytokine which has many biological functions, and it makes many important roles in tumor growth, proliferation, differentiation, apoptosis and so on. There are two types of TNF: TNF-α and TNF-β . The function of TNF- a is obviously stronger than TNF-β in induce tumor apoptosis. The biological properties of TNF are signaled through its cell surface receptors-TNFR (tumor necrosis factor receptor). Two different TNFRs were found: TNFR- I and TNFR- II. The cytotoxicity of TNF- a is mainly mediated by TNFR- I . Though there are many researches about using TNF to therapy CCA, the relations between TNF and CCA are not clear.Objective: To investigate the expression of TNF- a and TNFR-1 in CCA tissues and cyst of bile duct tissues, and the relations between the expression and the classify, invasion, differentiation of CCA. Method: We immunohistochemically analyzed the TNF- a and TNFR-1 expression in tissues from resected CCA and cyst of bile duct. Result: TNF- a mainly expressed in cytoplasm, and TNFR- I expressed in both cytoplasm and cytomembrane. The positive rate of TNF- a expression in CCA is 25%( 12/48), and the rate of TNFR- I is 75%(36/48). There is significant difference between two results (P<0.005). The expression of TNF- a is not correlated with thetumors differentiated, depth of invasion and whether or not lymphnode metastatic( P> 0.05). There is also no significant difference between the TNFR- I expression and tumor dedifferentiation, metastasis( P>0.05). But TNFR- I expressed significantly difference in depth of invasion( P< 0.005). In the tissues of cyst of bile duct, the positive rate of TNF- a is 5.3%, the positive of TNFR- I is 42.1%. The expression of TNF- a and TNFR- I in cyst of bile duct is lower than CCA( P<0.05, 0.005). Conclusion: 1. CCA tissues can express TNF- a and TNFR- I . 2. The expression of TNF- a in CCA is not correlated with the tumors differentiated, depth of invasion and lymphnode metastasis. TNFR- I expressed no significant difference between tumor differentiation and metastasis, but expressed significantly difference in depth of invasion. 3. TNF-α expressed obviously lower level than TNFR- I in CCA. 4. The expression of TNF- α and TNFR- I in CCA is higher than cyst of bile duct.