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目的观察脐带间充质干细胞(HUC-MSCs)移植联合血肿抽吸治疗大鼠脑出血后脑组织中p53基因的表达及大鼠神经功能恢复的情况。方法体外分离HUC-MSCs,移植前以BrdU标记。向尾状核注入2μLⅣ型胶原酶建立脑出血模型,将120只造模成功的SD大鼠随机分为脑出血组、血肿抽吸组、细胞移植组、细胞移植联合血肿抽吸组(联合治疗组),进行相应的处理后,于1、3、7、14和30 d 5个时间点分批处死大鼠,取脑组织,采用免疫组织化学法检测大鼠脑出血周围p53基因的表达及HUC-MSCs在脑组织的分布,并用改良的大鼠神经功能缺损评分(mNSS)评价大鼠的神经功能。结果与脑出血组比较,血肿抽吸组、细胞移植组和联合治疗组的mNSS评分、p53表达量均下降(P均<0.05);7 d时,联合治疗组的mNSS评分及p53表达量低于脑出血组和血肿抽吸组(P均<0.05),14 d、30 d时则低于其余3组(P均<0.05)。结论 HUC-MSCs移植联合血肿抽吸治疗可以显著减少大鼠脑出血后神经元的损伤,更有利于神经功能的恢复。
Objective To observe the expression of p53 gene and the recovery of neurological function in rat brain after intracerebral hemorrhage with transplantation of umbilical cord mesenchymal stem cells (HUC-MSCs) and hematoma aspiration. Methods HUC-MSCs were isolated in vitro and labeled with BrdU before transplantation. The model of intracerebral hemorrhage was established by injecting 2μL type Ⅳ collagenase into the caudate nucleus. 120 successful SD rats were randomly divided into cerebral hemorrhage group, hematoma aspiration group, cell transplantation group, cell transplantation group and hematoma aspiration group The rats were sacrificed at 1, 3, 7, 14 and 30 days after operation. The brain tissues were harvested and the expression of p53 gene in the perihematoma brain was detected by immunohistochemistry. The distribution of HUC-MSCs in brain tissue and the neurological function of rats were evaluated using a modified rat neurological deficit score (mNSS). Results Compared with ICH group, mNSS score and p53 expression in hematoma aspiration group, cell transplantation group and combined treatment group decreased (all P <0.05). On the 7th day, mNSS score and p53 expression in combination group were lower In the cerebral hemorrhage group and the hematoma aspiration group (all P <0.05), it was lower than the other three groups on the 14th and 30th days (P <0.05). Conclusion HUC-MSCs transplantation combined with hematoma aspiration therapy can significantly reduce the neuronal damage after intracerebral hemorrhage in rats and is more conducive to the recovery of neurological function.