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为了明确二斑叶螨Tetranychus urticae Koch对甲氰菊酯产生抗性的机理,在室内模拟田间药剂的选择压力,用甲氰菊酯对二斑叶螨敏感品系(S)进行逐代汰选,选育至38代时,获得了抗性倍数(resistance ratio,RR)为247.35的抗甲氰菊酯品系(Fe-R)。对S和Fe-R解毒酶活性的分析表明,Fe-R_(38)体内羧酸酯酶(carboxylesterase,CarE)、酸性磷酸酯酶(acid phosphatase,ACP)、碱性磷酸酯酶(alkaline phosphatase,ALP)、谷胱甘肽-S-转移酶(glutathione stransferase,GSTs)和多功能氧化酶(mixed function oxidase,MF0)较S体内相应酶的活力显著升高(p<0.05),其相对比值(R/S)分别为1.822,13.941,3.789,4.262和17.386。此外,筛选至第9,19,25,32代时,除Fe-R_(25)和Fe-R_(32)的MFO活性与S相比有显著性差异(P<0.05)外,其余解毒酶(CarE,ACP,ALP,GSTs)的活性与S相比均无显著性差异(P>0.05)。筛选至第38代时,5种解毒酶的活力与S相比均差异显著(P<0.05)。结果说明二斑叶螨Fe-R随着筛选代数的增加(第25代后),MFO活性的上升可能是二斑叶螨对甲氰菊酯产生抗性的主要原因。
In order to clarify the mechanism of Tetranychus urticae Koch’s resistance to fenpropathrin, the selection pressure of field agents was simulated indoors, and the strain of Tetranychus urticae Koch At 38 generations, an anti-fenvalerate line (Fe-R) with a resistance ratio (RR) of 247.35 was obtained. The analysis of the activity of S and Fe-R detoxification enzymes showed that the activity of carboxylesterase (CarE), acid phosphatase (ACP), alkaline phosphatase ALP, glutathione-S-transferase (GSTs) and mixed function oxidase (MFO) were significantly higher than those of the corresponding enzymes in S (p <0.05) R / S) were 1.822, 13.941, 3.789, 4.262 and 17.386, respectively. In addition, except the MFO activities of Fe-R_ (25) and Fe-R_ (32) were significantly different from those of S (P <0.05) (CarE, ACP, ALP, GSTs) activity compared with the S no significant difference (P> 0.05). From the 38th generation to the 38th generation, the activities of five detoxification enzymes were significantly different from those of S (P <0.05). The results showed that the two-spotted spider mite Fe-R with screening algebraic increase (after the 25th generation), the increase of MFO activity may be two-spotted spider mite resistant to fenpropathrin the main reason.