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目的观察降压通络方对高血压病肾损害大鼠肾脏肾上腺髓质素(ADM)表达的影响,探讨其对高血压肾损伤大鼠肾脏的保护作用。方法采用16周龄自发性高血压大鼠(SHR)为研究对象,将其随机分为模型组、缬沙坦组、降压通络方组、并设正常血压大鼠(WKY)为空白对照。降压通络方组、缬沙坦组分别给予降压通络方14.2 g/(kg·d)和缬沙坦10 mg/(kg·d)灌胃治疗,模型组及正常对照组给予等量的生理盐水。观察各组大鼠在给药前、给药2周、4周、8周后血压的变化,测定大鼠24 h尿蛋白定量,同时观察肾功能及肾脏病理组织学改变,检测大鼠肾脏中ADM的蛋白含量,并与大鼠的血压进行相关性分析。结果模型组及各治疗组大鼠血压、24 h尿蛋白、血肌酐(Scr)、尿素氮(BUN)较正常对照组明显升高,降压通络方组、缬沙坦组均明显低于模型组(P<0.01),但2个治疗组之间比较无显著性差异;模型及各治疗组大鼠肾脏ADM蛋白含量较正常对照组明显降低(P<0.01),与模型组比较,降压通络方及缬沙坦组大鼠肾脏ADM蛋白含量明显升高(P<0.01),并与大鼠的血压呈明显负相关(γ=-0.740,P<0.001),但2个治疗组之间比较无明显差异。HE染色显示降压通络方能显著改善肾小动脉及肾小球硬化,促进肾组织结构的恢复。结论降压通络方通过上调高血压肾损害大鼠肾脏ADM的表达,扩张肾脏局部小血管,改善肾脏缺血,在保护肾脏功能方面起到重要作用。
Objective To observe the effect of Antihypertension Tongluo Prescription on the expression of renal adrenomedullin (ADM) in hypertensive kidney lesion rats and to explore its protective effect on kidney in hypertensive renal lesion rats. Methods 16-week-old spontaneously hypertensive rats (SHR) were randomly divided into model group, valsartan group and JSTL group. Normal rats (WKY) were used as blank control . The antihypertensive Tongluo Recipe group and the valsartan group were administered with 14.2 g / (kg · d) of Xiaadong Tongluo Recipe and 10 mg / (kg · d) valsartan respectively. The model group and the normal control group were given The amount of saline. The changes of blood pressure before treatment, 2 weeks, 4 weeks and 8 weeks after administration were observed. The proteinuria of 24 hours urinary protein in rats was observed. At the same time, renal function and renal histopathological changes were observed. ADM protein content, and with the blood pressure in rats correlation analysis. Results The blood pressure, 24 h urine protein, serum creatinine (Scr) and blood urea nitrogen (BUN) in model group and each treatment group were significantly higher than those in normal control group, and were significantly lower than those in JSTT group and Valsartan group Model group (P <0.01), but there was no significant difference between the two treatment groups. The content of ADM protein in the model and each treatment group was significantly lower than that in the normal control group (P <0.01). Compared with the model group, The contents of ADM protein in VTR and Valsartan groups were significantly increased (P <0.01), and were negatively correlated with the blood pressure of rats (γ = -0.740, P <0.001) No significant difference between the comparison. HE staining showed that JSTT could significantly improve renal arterioles and glomerulosclerosis and promote the recovery of renal tissue structure. Conclusion HBJF can play an important role in protecting renal function by up-regulating the expression of ADM in the kidney of hypertensive rats and expanding the small local blood vessels in the kidneys to improve renal ischemia.