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用反转录聚合酶链反应（ＲＴ－ＰＣＲ），从纯化的腺胃病变型鸡传染性支气管炎分离株病毒基因组ＲＮＡ扩增出约１．７ｋｂ的ＩＢＶＳ１基因。将该扩增产物于ＨｉｎｄⅢ和ＢａｍＨⅠ位点克隆到ｐＵＣ１８质粒载体中，对重组质粒载体进行酶切分析，得到与Ｓ１基因预期大小一致的插入片断。经Ｓ１探针Ｓｏｕｔｈｅｒｎ杂交检测，表明插入片断为Ｓ１基因。利用该法筛选到４株阳性重组质粒转化子。
An approximately 1.7 kb IBVS1 gene was amplified from the viral genome RNA of a purified gD-type avian infectious bronchitis isolate using reverse transcription polymerase chain reaction (RT-PCR). The amplified product was cloned into pUC18 plasmid vector at HindIII and BamHI sites, and the recombinant plasmid vector was digested with restriction enzyme to obtain the inserted fragment with the expected size of S1 gene. S1 probe Southern hybridization test showed that the inserted fragment is S1 gene. Using this method, four positive recombinant plasmid transformants were screened.