在50年代 Hamlynu 和 Causeyc 就作了家兔颈上交感神经节(SCG)的电镜观察。由于受当时电镜分辨率(100 )所限,发表的图片不够清晰。Richardsan(1966)发现高锰酸钾固定法能用于区分外周神经系统中肾上腺能及胆碱能神经末梢,从而推进了神经细胞化学的发展。在以后十年里它成为常规方法,广泛用于中枢和外周神经系统胺能细胞 In the 1950s, Hamlynu and Causeyc made an electron microscopic observation of the rabbit cervical sympathetic ganglia (SCG). Due to the resolution of the electron microscope at that time (100), published pictures were not clear enough. Richardsan (1966) found that potassium permanganate fixation can be used to differentiate adrenal and cholinergic nerve endings in the peripheral nervous system, thereby promoting the development of neurocytochemistry. It became the routine method in the next ten years and is widely used in central and peripheral nervous system amnestic cells