目的建立高效液相色谱法测定硫鸟嘌呤原料及片剂的含量及有关物质。方法采用AltimaC18色谱柱,以0.05mol·L-1磷酸二氢钠溶液(pH3.0)为流动相,柱温为35℃;检测波长248nm;流速1.0mL·min-1。结果硫鸟嘌呤在8～120mg·L-1内线性关系良好(r=0.9999),最低检测限为0.12mg·L-1,回收率为99.27%。结论本方法准确、灵敏,精密度高、专属性强,可用于硫鸟嘌呤含量及有关物质的测定。 Objective To establish a HPLC method for the determination of thioguanine and its related substances. Methods Altima C18 column was used. The mobile phase was 0.05 mol·L-1 sodium dihydrogen phosphate solution (pH 3.0). The column temperature was 35 ℃. The detection wavelength was 248 nm. The flow rate was 1.0 mL · min-1. Results Thioguanine showed good linearity (r = 0.9999) in 8-120 mg · L-1 and the lowest detection limit was 0.12 mg · L-1. The recovery rate was 99.27%. Conclusion The method is accurate, sensitive, accurate and specific. It can be used for the determination of thioguanine and related substances.