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目的:探讨鼻咽癌组织p16、nm23基因蛋白表达与E1B-55K缺陷型腺病毒(简称H101)疗效关系。方法:经鼻咽部肿物活检确诊为鼻咽低分化鳞癌的病人接受化疗及颈部转移灶注射H101。应用免疫组化法检测鼻咽癌组织p16、nm23基因蛋白表达,并分析其与H101注射灶疗效的相关性。结果:30例鼻咽癌组织中p16、nm23基因蛋白表达阳性率分别为30.0(9/30)、83.3(25/30)。H101局部注射治疗的颈部肿物有效率为83.3(25/30)。p16基因蛋白表达阳性患者有效率55.6,阴性患者有效率95.2,其差异有统计学意义(P<0.01);nm23基因蛋白表达阳性患者有效率84.0,nm23阴性患者有效率80.0,其差异无统计学意义(P>0.05)。结论:鼻咽癌p16基因异常表达可能是H101作用机制的一个重要因素,检测p16基因有助于预测鼻咽癌患者对H101治疗的敏感性。
Objective: To investigate the relationship between the expression of p16 and nm23 protein in nasopharyngeal carcinoma and the efficacy of E1B-55K-deficient adenovirus (H101). Methods: Patients diagnosed as poorly differentiated squamous cell carcinoma of the nasopharynx by nasopharyngeal biopsy underwent chemotherapy and neck metastases injection H101. Immunohistochemistry was used to detect the protein expression of p16 and nm23 in nasopharyngeal carcinoma, and the correlation between p16 and nm23 gene expression and therapeutic effect of H101 injection was analyzed. Results: The positive rates of p16 and nm23 protein in 30 cases of nasopharyngeal carcinoma were 30.0 (9/30) and 83.3 (25/30), respectively. H101 local injection of neck mass treatment efficiency was 83.3 (25/30). The positive rate of p16 gene protein was 55.6% in the positive group and 95.2% in the negative group, the difference was statistically significant (P <0.01); the effective rate of the positive expression of nm23 protein was 84.0% and the negative rate of nm23 was 80.0 Significance (P> 0.05). Conclusion: Abnormal expression of p16 gene in nasopharyngeal carcinoma may be an important factor in the mechanism of action of H101. Detection of p16 gene may be helpful to predict the sensitivity of H101 treatment to patients with nasopharyngeal carcinoma.