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目的 了解G蛋白β3 亚基 (GNB3)基因C82 5T多态性与北京地区缺血性脑卒中发病之间的关系。方法 利用PCR和分子杂交技术对北京地区 2 94例缺血性脑卒中患者及 2 80例非脑卒中患者的C82 5T多态性进行检测和分析。结果 C82 5T多态性位点在两组人群中的分布均符合Hardy -weinberg遗传平衡定律 ,缺血性脑卒中患者中CC、CT、TT三种基因型频率分布依次为 30 .2 7%、48.98%、2 0 .75 % ,非脑卒中患者中为 33 .2 1%、47.5 0 %、19.2 9% ,两组人群中 82 5T等位基因频率分别为 0 .45 2和 0 .430 ,均无显著性差异。结论 GNB3基因C82 5T多态性与北京地区缺血性脑卒中发病无关。
Objective To understand the relationship between G82β gene polymorphism of GNB3 gene and ischemic stroke in Beijing. Methods PCR and molecular hybridization were used to detect and analyze the C82 5T polymorphism in 2 94 ischemic stroke patients and 280 non-stroke patients in Beijing. Results The distribution of C82 5T polymorphism loci was in line with the Hardy-weinberg law of genetic balance. The frequencies of CC, CT and TT genotypes in ischemic stroke patients were 30.27% 48.98%, 20.7% in non-stroke patients, 33.21%, 47.5%, 19.29% in non-stroke patients. The frequencies of 82 5T allele in the two groups were 0.452 and 0.304, No significant difference. Conclusion The C82 5T polymorphism of GNB3 gene is not related to the incidence of ischemic stroke in Beijing.