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目的探讨RNA干扰技术抑制SHBG基因表达对滋养细胞增殖的影响。方法在体外通过RNA干扰技术,将SHBG si RNA转染至原代培养的人绒毛滋养细胞中,MTT法检测滋养细胞增殖情况。结果成功将SHBG si RNA转染人绒毛滋养细胞;转染后24、48、72h,SHBG si RNA转染组的细胞生长速度显著减缓,正常对照组(未经转染的细胞)、空白对照组(只转染脂质体,无si RNA)和阴性对照组(转染非特异性si RNA)的组间比较差异均无统计学意义(P>0.05);转染组(SHBG si RNA-I,SHBG si RNA-II,SHBG si RNA-III)之间差异均无统计学意义(P>0.05),各转染组分别与各对照组的组间比较差异均有统计学意义(P<0.05)。结论 si RNA可以有效干扰人绒毛滋养细胞SHBG基因的表达,抑制滋养细胞增殖的增殖活性。
Objective To investigate the effect of RNA interference on the proliferation of trophoblasts induced by SHBG gene. METHODS: SHBG si RNA was transfected into primary culture human hair trophoblast cells by RNA interference technique in vitro. The proliferation of trophoblasts was detected by MTT assay. Results SHBG si RNA was successfully transfected into human villous trophoblast cells. After 24, 48 and 72 hours of transfection, the cell growth rate of SHBG si RNA transfection group was significantly slowed down. In normal control group (untransfected cells) and blank control group The expression of SHBG si RNA-I and SHBG si RNA-I in the transfected group was significantly lower than that in the untransfected group SHBG si RNA-II, SHBG si RNA-III) were not significantly different (P> 0.05), but there was significant difference between the two groups (P <0.05) . Conclusion si RNA can effectively interfere with the expression of human hair trophoblast SHBG gene and inhibit the proliferative activity of trophoblast cells.