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本项研究,革新了我国沿用几十年的白僵菌菌剂固体培养的方法,建立了液固两相一体化生产新工艺。调整培养基浓度,采用发酵罐生产菌体,发酵20小时左右即可放罐,用C-4为载体吸附完成发酵后的菌液,不补充任何营养物质和灭菌,经6天培养即可完成整个产孢过程。比传统生产方法缩短一半时间(7天)。用上述方法生产的原菌粉不需粉碎,含孢量达150～200亿/克。采用全密闭式抽风负压分离技术生产的高孢子粉含量平均为1000亿/克。孢子收回率达88％,这一套新工艺研制成功,将为较大规模生产白僵菌制剂提供基本条件。
This study has revolutionized the method of solid culture of Beauveria bassiana for decades in our country and established a new process of integrated liquid-solid two-phase production. Adjust the medium concentration, fermentor production of bacteria, fermentation can be placed about 20 hours, with C-4 as the carrier after the completion of fermentation of bacteria, without any nutrients and sterilization, after 6 days of culture Complete sporulation process. Shorten the time than traditional production methods by half (7 days). The original powder produced by the above method does not need to be smashed, and the spore content reaches 150 to 20 billion / g. The use of fully enclosed vacuum negative pressure separation technology to produce high spore powder content of an average of 100 billion / g. Spore recovery rate of 88%, the successful development of a new process will provide the basic conditions for the larger-scale production of Beauveria bassiana preparations.